Molecular screening for Candida orthopsilosis and Candidametapsilosis among Danish Candida parapsilosis group blood cultureisolates: proposal of a new RFLP profile for differentiation
نویسندگان
چکیده
منابع مشابه
Comparison of killing activity of caspofungin against Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis.
methods. In the other instance, a BLPAR strain was designated ampicillin-susceptible by Etest (MIC 1⁄4 1 mg/L), ampicillinintermediate by M.I.C.E. (MIC 1⁄4 2 mg/L) and ampicillin-resistant by BMD (MIC 1⁄4 16 mg/L). As stated earlier, the low MICs when gradient methods were used were because of a faint growth within the inhibition zone that was difficult to detect at 24 h. In contrast to the fin...
متن کاملEffect of usnic acid on Candida orthopsilosis and C. parapsilosis.
The activity of usnic acid against Candida orthopsilosis and Candida parapsilosis on planktonic and biofilm conditions was investigated by using a broth microdilution and microplate methods. Potent in vitro activities against different Candida species were obtained. The metabolic activity of sessile cells of C. parapsilosis complex was reduced by 80% at four times the 80% inhibitory concentrati...
متن کاملRapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp.
A 30-min fluorogenic test was developed for differentiation of members of the Candida parapsilosis group from other Candida species commonly encountered in clinical material. The fluorogenic substrate, 4-methylumbelliferyl-beta-D-glucoside, was utilized to assay beta-glucosidase activity. A total of 50 C. parapsilosis isolates and 135 isolates of four other Candida species were tested. Assay se...
متن کاملPyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.
Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between ...
متن کاملIn vitro and in vivo effects of echinocandins against Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis.
OBJECTIVES The aim of the present study was to compare, in vitro and in vivo, the effects of caspofungin, micafungin and anidulafungin against Candida parapsilosis complex isolates. METHODS In vitro activities of all three echinocandins were assessed against C. parapsilosis sensu stricto (n = 4), Candida orthopsilosis (n = 4) and Candida metapsilosis (n = 3) using broth microdilution suscepti...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2010
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.017293-0